Comparison of Cholesterol Levels Using Serum and Plasma Samples of EDTA Anticoagulants Using the CHOD-PAP Method
Health laboratory services or clinics can assist with patient recovery monitoring or diagnosis. The measurement of cholesterol levels is one of the frequent laboratory examinations. The CHOD-PAP enzymatic approach is the most used way to check your cholesterol levels. Plasma or serum is the test substance for cholesterol. Due to the presence of EDTA anticoagulant particles that will impact the test, the use of plasma is vulnerable to mixing with erythrocytes. This study used blood and plasma samples of EDTA anticoagulant to ascertain the variation in the outcomes of the average cholesterol levels. This research method compares two samples of EDTA anticoagulant—serum and plasma—in an analytical analysis using a comparative approach. According to the findings, there was a 1.12 percent difference between the average cholesterol levels in the serum sample (195.33 mg/dL) and the EDTA anticoagulant plasma sample (193.13 mg/dL). These findings indicate that there is no discernible difference in the cholesterol examination results obtained using EDTA anticoagulant-infused serum and plasma samples.
Boshtam, K.M., & Mayes P.A. (2006). Transport and storage of lipids. In: Wulandari N, Rendy L, Dwijayanthi L, Liena, Dany L, Rachman LY, ed. Harper's Biochemistry. 27th ed. Jakarta: EGC.
Hardisari, R., & Koriyah, B. (2016). Description of triglyceride levels (GPO-PAP method) in EDTA serum and plasma samples. Journal of Laboratory Technology March, (5), 27-31.
Jingbo, H, & Jiri, F. (2011). The impact of dietary changes and dietary supplements on lipid profile. Canadian Journal of Cardiology, 27, 488-505.
Kapoor, R. et al. (2015). A Leep Above Friedwald Formula for Calculation of Low Density Lipoprotein Cholesterol. Journal of Laboratory Physicians, 7 (1).
Kurniadi, H., & Nurrahmani U. (2014). Stop the symptoms of coronary heart disease, high cholesterol, diabetes mellitus, hypertension. Media Palace.
Molavi, F. et al. (2020). Comparison Common Equations For LDL-C Calculation With Direct Assay and Developing A Novel Formula In Iranian Children and Adolescents: The CASPIAN V Study. Journal of Lipids in Health and Disease, 19(1).
Philip, B., Antonio, MG., John, CL, Jaman, M., Michael, S., Scott, MG., John, JP., Vera, B., & Jean-Charles, F. (2007). HDL Cholesterol, Very Low Levels of LDL Cholesterol, and Cardiovascular Events. The New England Journal of Medicine, 357(13), 1301-1310.
Razi, F. et al. (2017). LDL-Cholesterol Measurement in Diabetic Type 2 Patiens: A Comparison between Direct Assay and Popular Equations. Journal of Diabetes and Metabolic, 16(1).
Sahebkar, A. (2013). Fat lowers fat: purified phospholipids as emerging therapies for dyslipidemia. Biochimica et Biophysica Acta (BBA)-Molecular and Cell Biology of Lipids, 1831(4), 887–893.
Sari, I., Sukeksi, A., & Ariyadi, T. (2017). Differences in SGPT levels of EDTA plasma and serum samples. Thesis. Semarang: Muhammadiyah University of Semarang;.
Vujovic, A. et al. (2010). Evaluation of different formulas for LDL-C calculation. Lipids in Health and Disease, 9(27), 1-9.
Warade, JP. (2016). Comparison between Direct Estimation of LDL and Friedwald's Formula. International Journal of Biomechemistry and Biophysics, 8(3), 41-46.
Widada, S. T., Martsiningsik, M.A., Carolina, S.C. (2016) Overview of differences in total cholesterol levels using the CHOD-PAP (Cholesterol Oxidase – Peroxidase Aminoantypirin) serum sample and EDTA plasma sample. Journal of Laboratory Technology. 5(1), 41-4.
Winarni, A., Anggraini, H., & Ariyadi, T. (2011). Differences in triglyceride levels in serum and plasma samples EDTA enzymatic method. Scientific papers. Semarang: Muhammadiyah University of Semarang.
World Health Organization. (2005). Promoting Mental Health: Concepts, Emerging evidence, Practice: A report of the World Health Organization, Department of Mental Health and Substance Abuse in collaboration ith the Victorian Health Promotion Foundation and the University of Melbourne. Geneva: World Health Organization.
Zhen, YC., Ka, YM., & Yintong, L. (2011). Role and classification of cholesterol lowering functional foods. Journal of Functional Foods, 3(6), 1-9.
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.